Ukwelashwa kweseli okuzenzakalelayo okuvelayo kusetshenziswa i-plasma ecebile nge-platelet (PRP) kungase kudlale indima eyisizayo ezinhlelweni ezihlukahlukene zokwelashwa kwemithi evuselelayo.Kunesidingo somhlaba wonke esingafezeki samasu okulungisa izicubu zokwelapha iziguli ezine-musculoskeletal (MSK) nezifo zomgogodla, i-osteoarthritis (OA) kanye namanxeba angapheli ayinkimbinkimbi kanye ne-refractory.Ukwelashwa kwe-PRP kusekelwe eqinisweni lokuthi i-platelet growth factor (PGF) isekela ukuphulukiswa kwesilonda nokulungisa i-cascade (ukuvuvukala, ukwanda nokulungiswa kabusha).Inani lezinhlobo ezahlukene ze-PRP zihlolwe kusukela ezifundweni zabantu, in vitro kanye nezilwane.Kodwa-ke, izincomo ze-in vitro kanye nezifundo zezilwane ngokuvamile ziholela emiphumeleni yomtholampilo ehlukene, ngoba kunzima ukuhumusha imiphumela yocwaningo olungezona ezokwelashwa kanye nezincomo zendlela ekwelapheni komtholampilo womuntu.Eminyakeni yamuva nje, kuye kwenziwa inqubekelaphambili ekuqondeni umqondo wobuchwepheshe be-PRP nama-agent we-biological, futhi imiyalelo emisha yocwaningo kanye nezinkomba ezintsha ziye zahlongozwa.Kulesi sibuyekezo, sizoxoxa ngenqubekelaphambili yakamuva ekulungiseleleni nasekubunjweni kwe-PRP, okuhlanganisa umthamo we-platelet, umsebenzi we-leukocyte kanye nomthethonqubo we-immune ongenayo futhi oguquguqukayo, umphumela we-5-hydroxytryptamine (5-HT) kanye nokukhulula ubuhlungu.Ngaphezu kwalokho, sixoxe ngendlela ye-PRP ehlobene nokuvuvukala kanye ne-angiogenesis ngesikhathi sokulungiswa kwezicubu nokuvuselelwa.Ekugcineni, sizobuyekeza imiphumela yezinye izidakamizwa emsebenzini we-PRP.
I-autologous platelet-rich plasma (PRP) iwuketshezi oluyingxenye yegazi le-autologous peripheral ngemva kokwelashwa, futhi ukugcwala kweplatelet kungaphezulu kunesisekelo.Ukwelashwa kwe-PRP kusetshenziswe izinkomba ezihlukahlukene iminyaka engaphezu kwe-30, okuholela ekubeni nesithakazelo esikhulu emandleni e-PRP ezenzakalelayo emithi yokuvuselela.Igama elithi i-orthopedic biological agent lisanda kwethulwa ukwelapha izifo ze-musculoskeletal (MSK), futhi lizuze imiphumela ethembisayo emandleni okuvuselela ama-heterogeneous bioactive PRP cell mixs.Njengamanje, ukwelashwa kwe-PRP kuyindlela yokwelapha efanele enezinzuzo zomtholampilo, futhi imiphumela yesiguli ebikiwe iyakhuthaza.Kodwa-ke, ukungahambisani kwemiphumela yesiguli kanye nokuqonda okusha kuye kwabeka izinselele ekusebenzeni kokusetshenziswa komtholampilo kwe-PRP.Esinye sezizathu kungaba inombolo nokuhlukahluka kwezinhlelo ze-PRP kanye nohlobo lwe-PRP emakethe.Lawa madivayisi ahlukile ngokuya ngevolumu yokuqoqwa kwe-PRP nohlelo lokulungiselela, okuholela ezicini ezihlukile ze-PRP kanye nama-biological agents.Ukwengeza, ukuntuleka kokuvumelana mayelana nokumiswa kwesikimu sokulungiselela i-PRP kanye nombiko ogcwele wama-biological agents ekusetshenzisweni komtholampilo kuholele emiphumeleni yombiko engahambisani.Imizamo eminingi yenziwe ukuze kubonakale futhi kuhlukanise i-PRP noma imikhiqizo etholakala egazini ekusetshenzisweni kwemithi yokuvuselela.Ngaphezu kwalokho, okuphuma ku-platelet, njengama-lysates e-platelet yabantu, kuye kwahlongozwa ucwaningo lwe-orthopedic kanye ne-in vitro stem cell.
Enye yamazwana okuqala ku-PRP yanyatheliswa ku-2006. Ukugxila okuyinhloko kwalokhu kubuyekezwa kuwumsebenzi kanye nemodi yesenzo se-platelet, umphumela we-PRP esigabeni ngasinye se-cascade yokuphulukisa, kanye nendima eyinhloko ye-platelet-derived growth factor. ngezinkomba ezahlukahlukene ze-PRP.Esigabeni sokuqala socwaningo lwe-PRP, intshisekelo enkulu ku-PRP noma i-PRP-gel kwakuwukuba khona kanye nemisebenzi ethile yezinto ezimbalwa zokukhula kweplatelet (PGF).
Kuleli phepha, sizoxoxa kabanzi ngokuthuthukiswa kwakamuva kwezinhlayiya ezihlukahlukene ze-PRP kanye ne-platelet cell membrane receptors kanye nemiphumela yazo ekulawulweni kwamasosha omzimba angaphakathi futhi aguquguqukayo.Ukwengeza, indima yamangqamuzana ngamanye angase abe khona ku-vial yokwelapha ye-PRP kanye nethonya labo enkambisweni yokuvuselelwa kwezicubu kuzoxoxwa ngokuningiliziwe.Ukwengeza, inqubekelaphambili yakamuva ekuqondeni ama-PRP biological agents, umthamo we-platelet, imiphumela ethile yamangqamuzana egazi amhlophe athile, kanye nemiphumela yokuhlushwa kwe-PGF kanye nama-cytokines emiphumeleni yokudla okunomsoco we-mesenchymal stem cell (MSCs) izochazwa, kuhlanganise ne-PRP eqondise okuhlukile. izindawo zeseli nezicubu ngemuva kokudluliselwa kwesignali yeseli kanye nemiphumela ye-paracrine.Ngokufanayo, sizoxoxa ngendlela ye-PRP ehlobene nokuvuvukala kanye ne-angiogenesis ngesikhathi sokulungiswa kwezicubu nokuvuselelwa.Okokugcina, sizobuyekeza umphumela we-analgesic we-PRP, umphumela wezinye izidakamizwa emsebenzini we-PRP, kanye nenhlanganisela ye-PRP nezinhlelo zokuvuselela.
Izimiso eziyisisekelo zokwelashwa kwe-plasma ecebile ngamaplatelet
Amalungiselelo e-PRP aya ngokuya ethandwa futhi asetshenziswa kabanzi emikhakheni ehlukahlukene yezokwelapha.Isimiso esiyisisekelo sesayensi sokwelashwa kwe-PRP yukuthi umjovo wamaplatelet agxilile endaweni elimele ungase uqalise ukulungiswa kwezicubu, ukuhlanganiswa kwezicubu ezintsha ezixhumeneyo kanye nokwakhiwa kabusha kokujikeleza kwegazi ngokukhulula izici eziningi eziphilayo (izici zokukhula, ama-cytokines, ama-lysosomes) kanye amaprotheni okunamathela anesibopho sokuqalisa ukusabela kwe-hemostatic cascade.Ngaphezu kwalokho, amaprotheni e-plasma (isb. i-fibrinogen, i-prothrombin, ne-fibronectin) akhona ezingxenyeni ze-plasma ezimpofu (PPPs).Ukugxila kwe-PRP kungakhuthaza ukukhululwa kwe-hyperphysiological yezinto zokukhula ukuze kuqale ukuphulukiswa kokulimala okungapheli futhi kusheshise inqubo yokulungisa ukulimala okukhulu.Kuzo zonke izigaba zenqubo yokulungisa izicubu, izici ezihlukahlukene zokukhula, ama-cytokines kanye nabalawuli besenzo sendawo bakhuthaza imisebenzi eminingi eyisisekelo yamaseli ngokusebenzisa i-endocrine, i-paracrine, i-autocrine kanye nezinqubo ze-endocrine.Izinzuzo eziyinhloko ze-PRP zihlanganisa ukuphepha kwayo kanye nobuchwepheshe bokulungiselela obuhlakaniphile bemishini yamanje yokuhweba, engasetshenziswa ukulungisa ama-biological agents angasetshenziswa kabanzi.Okubaluleke kakhulu, uma kuqhathaniswa ne-corticosteroids evamile, i-PRP iwumkhiqizo ozenzakalelayo ongenamiphumela emibi eyaziwayo.Kodwa-ke, akukho mthethonqubo ocacile wefomula nokubunjwa kokubunjwa kwe-PRP okujova, futhi ukwakheka kwe-PRP kunezinguquko ezinkulu kumaplatelet, okuqukethwe kwamangqamuzana amhlophe egazi (WBC), ukungcola kwamangqamuzana egazi abomvu (RBC), kanye nokuhlushwa kwe-PGF.
Amagama e-PRP nokuhlukaniswa
Emashumini eminyaka, ukuthuthukiswa kwemikhiqizo ye-PRP esetshenziselwa ukugqugquzela ukulungiswa nokuvuselelwa kwezicubu bekuyinkambu yocwaningo ebalulekile ye-biomaterials nesayensi yezidakamizwa.I-cascade yokuphulukisa izicubu ihlanganisa abahlanganyeli abaningi, okuhlanganisa ama-platelet kanye nezici zabo zokukhula kanye nama-cytokine granules, amangqamuzana egazi amhlophe, i-fibrin matrix namanye ama-synergistic cytokines amaningi.Kule nqubo ye-Cascade, inqubo eyinkimbinkimbi yokuhlanganisa izokwenzeka, okuhlanganisa ukusebenza kweplatelet kanye nokuqina okulandelayo kanye no-α- Ukukhululwa kokuqukethwe kwezinhlayiya zeplatelet, ukuhlanganiswa kwe-fibrinogen (ekhishwe ngamaplatelet noma mahhala ku-plasma) kunethiwekhi ye-fibrin, kanye nokwakheka. i-platelet embolism.
I-PRP ye-"Universal" ilingisa ukuqala kokuphulukisa
Ekuqaleni, igama elithi “platelet-rich plasma (PRP)” lalibizwa ngokuthi i- platelet concentrate elalisetshenziswa ekwelapheni ukumpontshelwa igazi, futhi lisasetshenziswa nanamuhla.Ekuqaleni, le mikhiqizo ye-PRP yayisetshenziswa kuphela njenge-fibrin tissue adhesive, kuyilapho ama-platelet ayesetshenziselwa ukusekela i-fibrin polymerization eqinile ukuze kuthuthukiswe ukuvalwa kwezicubu, kunokuba njengesikhuthazi sokuphulukisa.Ngemuva kwalokho, ubuchwepheshe be-PRP benzelwe ukulingisa ukuqaliswa kwe-cascade yokuphulukisa.Kamuva, ubuchwepheshe be-PRP bafingqwa ngokusebenzisa amandla abo okwethula nokukhulula izici zokukhula endaweni encane yendawo.Lo mdlandla wokulethwa kwe-PGF uvamise ukufihla indima ebalulekile yezinye izakhi kulokhu kuphuma kwegazi.Lo mdlandla uqina nakakhulu ngenxa yokuntuleka kolwazi lwesayensi, izinkolelo ezingaqondakali, izithakazelo zezohwebo kanye nokuntuleka kokumiswa nokuhlukaniswa.
I-biology ye-PRP concentrate iyinkimbinkimbi njengegazi ngokwalo, futhi ingase ibe yinkimbinkimbi kunezidakamizwa zendabuko.Imikhiqizo ye-PRP yizinto eziphilayo eziphilayo.Imiphumela yohlelo lokusebenza lwe-PRP yomtholampilo incike ezicini zangaphakathi, ezivamile neziguquguqukayo zegazi lesiguli, okuhlanganisa nezinye izingxenye ezihlukahlukene zamaselula ezingase zibe khona kusampula ye-PRP kanye nemvelo encane yendawo ye-receptor, engaba sesimweni esibucayi noma esingapheli.
Isifinyezo samagama e-PRP adidayo kanye nesistimu yokuhlukanisa ehlongozwayo
Iminyaka eminingi, odokotela, ososayensi kanye nezinkampani baye bahlushwa ukungaqondi kahle kokuqala kanye nokukhubazeka kwemikhiqizo ye-PRP kanye nemibandela yabo ehlukene.Abanye abalobi bachaza i-PRP njenge-platelet-kuphela, kanti abanye babonisa ukuthi i-PRP iqukethe namangqamuzana egazi abomvu, amangqamuzana egazi amhlophe ahlukahlukene, i-fibrin namaprotheni e-bioactive ngokuhlushwa okukhulayo.Ngakho-ke, ama-agent amaningi e-PRP e-biological aye afakwa ekusebenzeni komtholampilo.Kuyadumaza ukuthi izincwadi ngokuvamile azinayo incazelo enemininingwane yezinto eziphilayo.Ukwehluleka kokumiswa kokulungiswa komkhiqizo kanye nokuthuthukiswa kwesistimu yokuhlukanisa okwalandela kwaholela ekusetshenzisweni kwenani elikhulu lemikhiqizo ye-PRP echazwe ngamagama ahlukene nezifinyezo.Akumangalisi ukuthi izinguquko emalungiselelweni e-PRP ziholela emiphumeleni yesiguli engahambisani.
U-Kingsley waqala ukusebenzisa igama elithi “plasma-rich rich plasma” ngo-1954. Eminyakeni eminingi kamuva, u-Ehrenfest et al.Uhlelo lokuqala lokuhlukanisa olusekelwe eziguquguqukayo ezintathu eziyinhloko (i-platelet, i-leukocyte kanye nokuqukethwe kwe-fibrin) kwahlongozwa, futhi imikhiqizo eminingi ye-PRP yahlukaniswa yaba izigaba ezine: i-P-PRP, i-LR-PRP, i-fibrin ecebile ye-platelet (P-PRF) ne-leucocyte. i-PRF ecebile (L-PRF).Le mikhiqizo ilungiswa ngesistimu evaliwe ezenzakalelayo ngokuphelele noma iphrothokholi eyenziwe ngesandla.Ngaleso sikhathi, u-Everts et al.Ukubaluleka kokukhuluma ngamaseli amhlophe egazi emalungiselelweni e-PRP kwagcizelelwa.Baphinde batusa ukusetshenziswa kwamagama afanele ukusho izinguqulo ezingasebenzi noma ezicushiwe zamalungiselelo e-PRP kanye nejeli yeplatelet.
Delong et al.ihlongoze uhlelo lokuhlukaniswa kwe-PRP olubizwa ngokuthi ama-platelet, amaseli amhlophe egazi acushiwe (PAW) ngokusekelwe enanini eliphelele lama-platelet, okuhlanganisa nezigaba ezine zokuhlushwa ze-platelet.Eminye imingcele ihlanganisa ukusetshenziswa kwama-activator e-platelet kanye nokuba khona noma ukungabi khona kwamangqamuzana amhlophe egazi (okungukuthi ama-neutrophils).UMishra et al.Kuphakanyiswa uhlelo olufanayo lokuhlukanisa.Ngemva kweminyaka embalwa, uMautner kanye nozakwabo bachaza uhlelo lokuhlukanisa olunemininingwane eminingi (PLRA).Umbhali ufakazele ukuthi kubalulekile ukuchaza inani eliphelele leplatelet, okuqukethwe kwamangqamuzana amhlophe egazi (okuhle noma okungalungile), amaphesenti e-neutrophil, i-RBC (enhle noma engalungile) kanye nokuthi kusetshenziswe i-exogenous activation.Ngo-2016, uMagalon et al.Ukuhlukaniswa kwe-DEPA okusekelwe kumthamo womjovo we-platelet, ukusebenza kahle kokukhiqiza, ukuhlanzeka kwe-PRP okutholiwe kanye nenqubo yokwenza kusebenze yanyatheliswa.Kamuva, u-Lana nozakwabo bethula uhlelo lokuhlukanisa lwe-MARSPILL, olugxile kumaseli egazi ayingxenye ye-mononuclear.Muva nje, i-Scientific Standardization Committee ikhuthaze ukusetshenziswa kohlelo lokuhlukanisa lwe-International Society for Thrombosis and Hemostasis, olusekelwe ochungechungeni lwezincomo zokuvumelana ukuze kumiswe ukusetshenziswa kwemikhiqizo ye-platelet ekufakweni kwemithi yokuvuselela, okuhlanganisa nemikhiqizo ye-platelet efriziwe nencibilikisiwe.
Ngokusekelwe ohlelweni lokuhlukaniswa kwe-PRP oluhlongozwe odokotela nabacwaningi abahlukahlukene, imizamo eminingi engaphumelelanga yokumisa ukukhiqizwa, incazelo kanye nefomula ye-PRP ezosetshenziswa odokotela ingenza isiphetho esifanelekile, okungenzeka ukuthi singenzeki eminyakeni embalwa ezayo Ngaphezu kwalokho. , ubuchwepheshe bemikhiqizo ye-PRP yomtholampilo buyaqhubeka nokuthuthukiswa, futhi idatha yesayensi ibonisa ukuthi amalungiselelo e-PRP ahlukene ayadingeka ukuze aphathe ama-pathologies ahlukene ngaphansi kwezimo ezithile.Ngakho-ke, silindele ukuthi imingcele kanye neziguquguqukayo zokukhiqizwa kwe-PRP ekahle kuzoqhubeka nokukhula esikhathini esizayo.
Indlela yokulungiselela i-PRP iyaqhubeka
Ngokusho kwamatemu e-PRP kanye nencazelo yomkhiqizo, amasistimu wokuhlukanisa amaningana akhululwa ekwenziweni okuhlukile kwe-PRP.Ngeshwa, akukho ukuvumelana ohlelweni olubanzi lokuhlukanisa lwe-PRP nanoma yiliphi elinye igazi elizenzakalelayo nemikhiqizo yegazi.Ngokufanelekile, uhlelo lokuhlukanisa kufanele lunake izici ezihlukahlukene ze-PRP, izincazelo kanye negama elifanele elihlobene nezinqumo zokwelashwa kweziguli ezinezifo ezithile.Njengamanje, izinhlelo zokusebenza zamathambo zihlukanisa i-PRP ngezigaba ezintathu: i-pure platelet-rich fibrin (P-PRF), i-leucocyte-rich PRP (LR-PRP) kanye ne-leucocyte-deficient PRP (LP-PRP).Nakuba icace kakhudlwana kunencazelo evamile yomkhiqizo we-PRP, izigaba ze-LR-PRP kanye ne-LP-PRP ngokusobala azinazo izinto ezicacile zokuqukethwe kwamangqamuzana egazi amhlophe.Ngenxa yezindlela zayo zokuzivikela zokuzivikela ezifweni, amangqamuzana amhlophe egazi athinte kakhulu i-intrinsic biology yezifo zezicubu ezingapheli.Ngakho-ke, ama-biological agents e-PRP aqukethe amangqamuzana athile amhlophe egazi angakhuthaza kakhulu ukulawulwa kokuzivikela komzimba kanye nokulungiswa kwezicubu nokuvuselelwa.Ngokuqondile, ama-lymphocyte maningi ku-PRP, akhiqiza isici sokukhula esifana ne-insulin futhi asekela ukulungiswa kabusha kwezicubu.
Ama-monocyte nama-macrophages adlala indima ebalulekile enqubweni yokulawulwa kokuzivikela komzimba kanye nendlela yokulungisa izicubu.Ukubaluleka kwama-neutrophils ku-PRP akucaci.I-LP-PRP yanqunywa njengelungiselelo lokuqala le-PRP ngokuhlolwa okuhlelekile ukuze kuzuzwe imiphumela yokwelapha ephumelelayo ye-OA ehlangene.Nokho, uLana et al.Ukusetshenziswa kwe-LP-PRP ekwelapheni i-OA yamadolo kuyaphikiswa, okubonisa ukuthi amangqamuzana athile egazi amhlophe adlala indima ebalulekile enqubweni yokuvuvukala ngaphambi kokuvuselelwa kwezicubu, ngoba akhulula ama-molecule aphikisana nokuvuvukala.Bathola ukuthi inhlanganisela yama-neutrophils namaplatelet acushiwe abe nemiphumela emihle kunemiphumela engemihle ekulungisweni kwezicubu.Baphinde baveza ukuthi i-plasticity ye-monocyte ibalulekile ekusebenzeni okungezona ukuvuvukala nokulungisa ekulungiseni izicubu.
Umbiko wohlelo lokulungiselela i-PRP ocwaningweni lomtholampilo awuhambisani kakhulu.Izifundo eziningi ezishicilelwe azizange ziphakamise indlela yokulungiselela i-PRP edingekayo ukuze kuphindwe kabusha uhlelo.Akukho ukuvumelana okucacile phakathi kwezinkomba zokwelashwa, ngakho-ke kunzima ukuqhathanisa imikhiqizo ye-PRP kanye nemiphumela yokwelashwa ehlobene nayo.Ezimweni eziningi ezibikiwe, ukwelashwa okuhlushwa kwe-platelet kuhlukaniswa ngaphansi kwegama elithi "PRP", ngisho nenkomba efanayo yomtholampilo.Kweminye imikhakha yezokwelapha (njenge-OA ne-tendinosis), inqubekelaphambili yenziwe ekuqondeni izinguquko zamalungiselelo e-PRP, imizila yokulethwa, umsebenzi weplatelet nezinye izingxenye ze-PRP ezithinta ukulungiswa kwezicubu nokuvuselelwa kwezicubu.Kodwa-ke, ucwaningo olwengeziwe luyadingeka ukuze kufinyelelwe esivumelwaneni mayelana namagama e-PRP ahlobene nama-PRP biological agents ukuze uphathe ngokugcwele nangokuphephile izifo ezithile nezifo.
Isimo sesistimu yokuhlukaniswa kwe-PRP
Ukusetshenziswa kwe-autologous PRP biotherapy kukhathazwa ukuhlukahluka kwamalungiselelo e-PRP, ukuqamba amagama okungahambisani nokumiswa okungalungile kwemikhombandlela esekelwe ebufakazini (okungukuthi, kunezindlela eziningi zokulungiselela ukukhiqiza izitsha zokwelashwa komtholampilo).Kungabikezelwa ukuthi okuqukethwe okuphelele kwe-PRP, ukuhlanzeka kanye nezici zebhayoloji ze-PRP nemikhiqizo ehlobene ziyahlukahluka kakhulu, futhi kuthinta ukusebenza kahle kwezinto eziphilayo kanye nemiphumela yokuhlolwa komtholampilo.Ukukhethwa kwedivayisi yokulungiselela i-PRP kwethula ukuguquguquka kokhiye wokuqala.Emithi yokuvuselela emtholampilo, odokotela bangasebenzisa izinto ezimbili ezihlukene zokulungiselela i-PRP nezindlela.Ukulungiswa kusebenzisa isihlukanisi esivamile samangqamuzana egazi, esisebenza egazini eliphelele eliqoqwe ngokwalo.Le ndlela isebenzisa isigubhu se-centrifuge esiqhubekayo sokugeleza noma ubuchwepheshe bokuhlukanisa idiski kanye nezinyathelo eziqinile nezithambile ze-centrifuge.Iningi lala madivaysi asetshenziswa ekuhlinzeni.Enye indlela ukusebenzisa ubuchwepheshe be-gravity centrifugal kanye nemishini.I-High G-force centrifugation isetshenziswa ukuhlukanisa ungqimba oluphuzi lwe-ESR neyunithi yegazi eliqukethe ama-platelet namaseli egazi amhlophe.Lawa madivaysi okugxilisa ingqondo mancane kunezihlukanisi zamaseli egazi futhi angasetshenziswa eduze kombhede.Umehluko ģ - Isikhathi sokuphoqa kanye ne-centrifugation siholela kumehluko omkhulu ekuvuneni, ekugxiliseni, ekuhlanzekeni, ekusebenzeni, nasekwenzeni kusebenze ama-platelet angawodwa.Izinhlobo eziningi zemishini yokulungiselela i-PRP yokuhweba ingasetshenziswa esigabeni sakamuva, okuholela ezinguqukweni ezengeziwe kokuqukethwe komkhiqizo.
Ukuntuleka kokuvumelana endleleni yokulungiselela kanye nokuqinisekiswa kwe-PRP kuyaqhubeka nokuholela ekungavumelani kokwelashwa kwe-PRP, futhi kukhona umehluko omkhulu ekulungiseleleni i-PRP, ikhwalithi yesampula kanye nemiphumela yomtholampilo.Imishini ye-PRP yezentengiselwano ekhona kakade iqinisekisiwe futhi yabhaliswa ngokuvumelana nemininingwane yomkhiqizi wobunikazi, oxazulula ukuguquguquka okuhlukile phakathi kwemishini ye-PRP etholakalayo njengamanje.
Qonda umthamo weplatelet in vitro kanye ne-vivo
Umphumela wokwelapha we-PRP kanye nokunye okugxilwe kweplatelet kubangelwa ukukhululwa kwezici ezihlukahlukene ezihilelekile ekulungiseni nasekuvuseleleni izicubu.Ngemuva kokusebenza kwama-platelet, ama-platelet azokwakha i-platelet thrombus, ezosebenza njenge-matrix yesikhashana ye-extracellular ukukhuthaza ukwanda kwamaseli nokuhlukaniswa.Ngakho-ke, kufanelekile ukucabanga ukuthi umthamo ophezulu we-platelet uzoholela ekugxilweni okuphezulu kwendawo kwezinto ze-platelet bioactive.Kodwa-ke, ukuhlobana phakathi komthamo kanye nokuhlushwa kwama-platelet kanye nokugxila kwe-platelet bioactive ukukhula factor kanye nesidakamizwa kungase kungalawuleki, ngoba kukhona umehluko omkhulu ekubalweni kweplatelet eyisisekelo phakathi kweziguli ngazinye, futhi kukhona umehluko phakathi kwezindlela zokulungiselela i-PRP.Ngokufanayo, izici eziningana zokukhula kweplatelet ezihilelekile endleleni yokulungisa izicubu zikhona engxenyeni ye-plasma ye-PRP (isibonelo, ukukhula kwesibindi kanye ne-insulin-like growth factor 1).Ngakho-ke, umthamo ophezulu weplatelet ngeke uthinte amandla okulungisa alezi zici zokukhula.
Ucwaningo lwe-in vitro PRP ludume kakhulu ngoba imingcele ehlukene kulezi zifundo ingalawulwa ngokunembile futhi imiphumela ingatholakala ngokushesha.Ucwaningo oluningana lubonise ukuthi amaseli asabela ku-PRP ngendlela encike kumthamo.U-Nguyen no-Pham babonise ukuthi ukugxila okuphezulu kakhulu kwe-GF kwakungahlosile ngempela inqubo yokuvuselela amaseli, okungenzeka kungasebenzi.Ezinye izifundo ze-in vitro zibonise ukuthi ukugxila okuphezulu kwe-PGF kungase kube nemiphumela emibi.Esinye isizathu kungaba inani elilinganiselwe lama-cell membrane receptors.Ngakho-ke, uma izinga le-PGF seliphezulu kakhulu uma liqhathaniswa nama-receptors atholakalayo, azoba nomthelela omubi ekusebenzeni kweseli.
Ukubaluleka kwedatha yokuhlushwa kweplatelet ku-vitro
Yize ucwaningo lwe-in vitro lunezinzuzo eziningi, luphinde lube nokubi okuthile.I-In vitro, ngenxa yokusebenzelana okuqhubekayo phakathi kwezinhlobo eziningi zamaseli ahlukene kunoma iyiphi izicubu ngenxa yesakhiwo sethishu nezicubu zamangqamuzana, kunzima ukuphindaphinda i-in vitro endaweni yesiko elinezinhlangothi ezimbili.Ukuminyana kwamaseli okungathinta indlela yesignali yeseli kuvame ukuba ngaphansi kuka-1% wesimo sethishu.Izicubu zesitsha esinezinhlangothi ezimbili zivimbela amaseli ukuthi angavezwa ku-extracellular matrix (ECM).Ngaphezu kwalokho, ubuchwepheshe obujwayelekile bamasiko buzoholela ekuqongeleleni udoti wamaseli kanye nokusetshenziswa okuqhubekayo kwezakhi.Ngakho-ke, isiko le-in vitro lihlukile kunoma yisiphi isimo sokuzinza, ukunikezwa kwe-oksijeni kwezicubu noma ukushintshaniswa okungazelelwe kwesiko lesiko, futhi imiphumela ephikisanayo ishicilelwe, kuqhathaniswa umphumela womtholampilo we-PRP nocwaningo lwe-in vitro lwamaseli athile, izinhlobo zezicubu kanye neplatelet. ukugxila.Graziani nabanye.Kutholakale ukuthi i-in vitro, umphumela omkhulu ekwandeni kwe-osteoblasts kanye ne-fibroblasts yafinyelelwa ekugxilweni kwe-PRP platelet izikhathi ezingu-2.5 ngaphezu kwenani lesisekelo.Ngokuphambene nalokho, idatha yomtholampilo ehlinzekwe yiPaki kanye nozakwethu ibonise ukuthi ngemva kokuhlanganiswa komgogodla, izinga leplatelet le-PRP lidinga ukunyuswa izikhathi ezingaphezu kwe-5 kunesisekelo sokuqala ukuze senze imiphumela emihle.Imiphumela efanayo ephikisanayo nayo yabikwa phakathi kwedatha yokwanda kwe-tendon in vitro kanye nemiphumela yomtholampilo.
(Okuqukethwe yile ndatshana kuphrintwe kabusha, futhi asinikezeli nganoma yisiphi isiqinisekiso esicacile noma esishiwoyo sokunemba, ukwethembeka noma ukuphelela kokuqukethwe okuqukethwe kulesi sihloko, futhi asinasibopho ngemibono yalesi sihloko, sicela uqonde.)
Isikhathi sokuthumela: Mar-01-2023