Isebenza kanjani i-PRP?

I-PRP yenza ngokuwohloka kwama-alpha granules asuka kumaplatelet, aqukethe izici ezimbalwa zokukhula.Ukukhishelwa okusebenzayo kwalezi zici zokukhula kuqalwa inqubo yokuhlangana kwegazi futhi kuqala phakathi nemizuzu eyi-10 yokuhlangana.Ngaphezu kuka-95% wezinto zokukhula ezenziwe ngaphambilini zifihliwe phakathi nehora elingu-1.Ngakho-ke, i-PRP kufanele ilungiswe esimweni se-anticoagulant futhi kufanele isetshenziswe ekuxhunyweni, ama-flaps, noma amanxeba kungakapheli imizuzu eyi-10 yokuphuma kwehlule.Izifundo ezingasebenzisi igazi eligcwele i-anticoagulated akuzona izifundo ze-PRP eziyiqiniso futhi ziyadukisa.

Njengoba ama-platelet enziwa asebenze inqubo yokujiyisa, izici zokukhula zifihliwe kusuka kuseli ngolwelwesi lwamaseli.Kule nqubo, izinhlayiya ze-alpha zihlangana kulwelwesi lwamaseli eplatelet, futhi izici zokukhula kwamaprotheni ziqeda isimo se-bioactive ngokwengeza amaketanga aseceleni e-histone nama-carbohydrate kulawa maprotheni.Ngakho-ke, ama-platelet awonakalisiwe noma angasebenzi ngokwelashwa kwe-PRP awakhiphi izici zokukhula kwe-bioactive futhi angase aholele emiphumeleni edumazayo.Izici zokukhula eziyimfihlo zibopha ngokushesha endaweni engaphandle yolwelwesi lwamaseli ku-graft, flap, noma inxeba ngama-transmembrane receptors.

Ucwaningo luye lwabonisa ukuthi amangqamuzana e-mesenchymal stem abantu abadala, ama-osteoblasts, ama-fibroblasts, amaseli e-endothelial, namaseli e-epidermal aveza ama-cell membrane receptors ezicini zokukhula ku-PRP.Lawa ma-receptors e-transmembrane nawo adala ukuthi kusebenze amaprotheni ophawu lwangaphakathi angaphakathi aholela ekukhulumeni (ukuvulwa) kokulandelana kwezakhi zofuzo ezijwayelekile zamangqamuzana, njengokwanda kwamaseli, ukwakheka kwe-matrix, ukwakheka kwe-osteoid, ukuhlanganiswa kwe-collagen, njll.

Ukubaluleka kwalolu lwazi ukuthi izici zokukhula kwe-PRP azilokothi zingene esitokisini noma ku-nucleus yayo, aziwona ama-mutagenic, zivele zisheshise ukukhuthazwa kokuphulukiswa okuvamile.Ngakho-ke, i-PRP ayinalo ikhono lokudala ukwakheka kwesimila.

Ngemva kokuqhuma kokuqala kwezici zokukhula ezihambisana ne-PRP, ama-platelet ahlangana futhi akhiphe izici ezengeziwe zokukhula ezinsukwini ezingu-7 ezisele zesikhathi sokuphila kwawo.Lapho ama-platelet esephelile futhi efile, ama-macrophage afinyelela esifundeni ngemithambo yegazi evuselelwe i-platelet akhulela ngaphakathi ukuze abambe iqhaza lokulawula ukuphulukiswa kwesilonda ngokufihla ezinye zezinto ezifanayo zokukhula kanye nezinye.Ngakho, inani lama-platelet ku-graft, inxeba, noma ihlule legazi elixhunywe ku-flap linquma ukuthi isilonda saphola ngokushesha kangakanani.I-PRP ivele yengeze kuleyo nombolo.

Mangaki ama-platelet anele?

Ucwaningo luye lwabonisa ukuthi ukwanda nokuhlukaniswa kwe-MSCS yabantu abadala kuhlobene ngokuqondile nokuhlushwa kwe-platelet.Babonise amajika okuphendula umthamo, okubonisa ukuthi impendulo eyanele yeselula ekugxilweni kweplatelet kwaqala lapho izikhathi ezine kuya kweziyisihlanu isibalo seplatelet eyisisekelo sifinyelelwa.Ucwaningo olufanayo lubonise ukuthi ukugxila kwe-platelet okwandayo kuphinde kwathuthukisa ukwanda kwe-fibroblast kanye nokukhiqizwa kwe-collagen yohlobo lwe-I, nokuthi iningi lempendulo lalincike ku-PH, nokuphendula okungcono kakhulu okwenzeka kumazinga e-pH ane-acidic eyengeziwe.

Lezi zifundo azibonisi nje isidingo samadivayisi ukuze agxilise amaplatelet anele, kodwa futhi achaze imiphumela ethuthukisiwe yokuvuselelwa kwamathambo kanye nemiphumela ethuthukisiwe yezicubu ezithambile ezihambisana ne-PRP.

Njengoba abantu abaningi benesibalo sesisekelo seplatelet esingu-200,000±75,000 nge-μl, isibalo seplatelet ye-PRP esiyisigidi esingu-1 nge-μl ngayinye elinganiswa nge-aliquots evamile engu-6-ml isibe inkomba “ye-PRP yokwelapha.”Okubalulekile, ucwaningo luye lwabonisa ukuthi lokhu kugxila kwe-platelet kufinyelelwa lapho amazinga okwelashwa efinyelelwa, ngaleyo ndlela kudedelwa izici zokukhula.

(Okuqukethwe yile ndatshana kuphrintwe kabusha, futhi asinikezeli nganoma yisiphi isiqinisekiso esicacile noma esishiwoyo sokunemba, ukwethembeka noma ukuphelela kokuqukethwe okuqukethwe kulesi sihloko, futhi asinasibopho ngemibono yalesi sihloko, sicela uqonde.)