Ama-platelet, njengezingcezu zamangqamuzana avela kumnkantsha we-megakaryocyte, abonakala ngokungabikho kwama-nuclei.I-platelet ngayinye iqukethe izinhlobo ezintathu zezinhlayiya, okuyi-α Granules, imizimba eminyene nama-lysosomes anenani elihlukile.Kubandakanya i-α Ama-granules acebile ngaphezu kwamaprotheni e-300 ahlukene, njenge-vascular endothelial activating factor, i-leukocyte chemotactic factor, i-activating factor, ukulungiswa kwezicubu ezihlobene nokukhula kwe-peptide kanye ne-antibacterial peptide, ehilelekile ezinqubweni eziningi ze-physiological and pathological, ezifana nokuphulukiswa kwesilonda. , i-angiogenesis kanye ne-anti-infection immunity.
Umzimba ominyene uqukethe ukugxila okuphezulu kwe-adenosine diphosphate (ADP), i-adenosine triphosphate (ATP), i-Ca2+, i-Mg2+ kanye ne-5-hydroxytryptamine.I-Lysosomes iqukethe izinhlobonhlobo ze-sugar proteases, njenge-glycosidase, i-proteases, amaprotheni e-cationic namaprotheni anomsebenzi we-bactericidal.Lezi GF zikhishelwa egazini ngemva kokusebenza kweplatelet.
I-GF icupha ukusabela kwe-cascade ngokubopha ngezinhlobo ezahlukene zama-membrane receptors, futhi ivule imisebenzi ethile kunqubo yokuvuselelwa kwezicubu.Njengamanje, i-GF efundwa kakhulu yi-platelet derived growth factor (PDGF) kanye ne-transforming growth factor (TGF- β (TGF- β) , I-Vascular endothelial growth factor (VEGF), i-epidermal growth factor (EGF), i-fibroblast growth factor (FGF), i-connective tissue growth factor (CTGF) kanye ne-insulin-like growth factor-1 (IGF-1). Lawa ma-GF asiza ukulungisa imisipha, i-tendon, ligament nezinye izicubu ngokugqugquzela ukwanda kwamaseli nokuhlukaniswa, i-angiogenesis nezinye izinqubo, bese zidlala okufanayo. indima.
Ukusetshenziswa kwe-PRP ku-Vitiligo
I-Vitiligo, njengesifo esivamile se-autoimmune, kanye nesifo sesikhumba esikhubazekile, sinomthelela omubi ku-psychology yeziguli futhi sithinte kakhulu izinga lempilo yeziguli.Ukufingqa, ukuvela kwe-vitiligo kuwumphumela wokusebenzelana kwezinto zofuzo kanye nezici zemvelo, okubangela ukuthi ama-melanocyte esikhumba ahlaselwe futhi alinyazwe yi-autoimmune system.Njengamanje, nakuba kunemithi eminingi yokwelapha i-vitiligo, ukusebenza kwayo ngokuvamile akubi kahle, futhi izindlela eziningi zokwelapha azinabo ubufakazi bomuthi osekelwe ebufakazini.Eminyakeni yamuva nje, ngokuhlola okuqhubekayo kwe-pathogenesis ye-vitiligo, ezinye izindlela zokwelapha ezintsha bezilokhu zisetshenziswa.Njengendlela ephumelelayo yokwelapha i-vitiligo, i-PRP isetshenziswe ngokuqhubekayo.
Njengamanje, i-308 nm excimer laser kanye ne-311 nm narrow band ultraviolet (NB-UVB) kanye nobunye ubuchwepheshe be-phototherapy bubonakala ngokuya busebenza ngempumelelo ezigulini ezine-vitiligo.Njengamanje, ukusetshenziswa komjovo we-autologous PRP subcutaneous microneedle kuhlanganiswe ne-phototherapy ezigulini ezine-vitiligo ezinzile kuye kwathuthuka kakhulu.Abdelghani et al.bathole ocwaningweni lwabo ukuthi umjovo we-autologous we-PRP we-microneedle ohlanganiswe ne-NB-UVB phototherapy unganciphisa kakhulu isikhathi sokwelashwa esiphelele seziguli ze-vitiligo.
Khattab et al.belaphe iziguli ezine-vitiligo ezinzile engeyona ingxenye ye-308 nm excimer laser ne-PRP, futhi bathole imiphumela emihle.Kutholwe ukuthi inhlanganisela yakho kokubili ingathuthukisa ngempumelelo izinga lombala we-leucoplakia, ifinyeze isikhathi sokwelashwa, futhi igweme ukusabela okubi kokusetshenziswa kwesikhathi eside kwe-308 nm excimer laser irradiation.Lezi zifundo ziphakamisa ukuthi i-PRP ehlanganiswe ne-phototherapy iyindlela ephumelelayo yokwelashwa kwe-vitiligo.
Kodwa-ke, u-Ibrahim kanye nezinye izifundo ziphakamisa ukuthi i-PRP iyodwa ayisebenzi ekwelapheni i-vitiligo.Kadry et al.yenza ucwaningo olulawulwa ngokungahleliwe mayelana nokwelashwa kwe-vitiligo nge-PRP ehlanganiswe ne-carbon dioxide dot matrix laser, futhi yathola ukuthi i-PRP ehlanganiswe ne-carbon dioxide dot matrix laser kanye ne-PRP iyodwa izuze umphumela omuhle wokukhiqiza umbala.Phakathi kwabo, i-PRP ehlangene ne-carbon dioxide dot matrix laser yayinomphumela omuhle kakhulu wokukhiqiza umbala, futhi i-PRP iyodwa yayizuze ukukhiqizwa kombala okulinganiselayo ku-leukoplakia.Umphumela wokukhiqiza umbala we-PRP uwodwa wawungcono kunalowo we-carbon dioxide dot matrix laser iyodwa ekwelapheni i-vitiligo.
Ukusebenza Kuhlanganiswe ne-PRP ekwelapheni i-Vitiligo
I-Vitiligo uhlobo lwesifo sombala esibonakala ngokukhipha umbala.Izindlela zokwelapha ezivamile zihlanganisa ukwelashwa kwezidakamizwa, i-phototherapy noma ukuhlinzwa, noma inhlanganisela yezindlela zokwelapha eziningi.Ezigulini ezine-vitiligo ezinzile kanye nomphumela omubi wokwelashwa okuvamile, ukwelashwa kokuhlinzwa kungaba ukungenelela kokuqala.
Garg et al.wasebenzisa i-PRP njenge-ejenti yokumiswa kwamaseli e-epidermal, futhi wasebenzisa i-Er: YAG laser ukugaya amabala amhlophe, okwazuza umphumela omuhle wokwelapha ekwelapheni iziguli ezizinzile ze-vitiligo.Kulolu cwaningo, iziguli ezingu-10 ezine-vitiligo ezinzile zabhaliswa futhi izilonda ezingu-20 zatholakala.Ezilonda ze-20, izilonda ze-12 (i-60%) zibonise ukubuyiswa okuphelele kwe-pigment, izilonda ze-2 (i-10%) zibonise ukubuyiswa kwe-pigment enkulu, izilonda ze-4 (20%) zibonise ukubuyiswa kwe-pigment okulinganiselwe, futhi izilonda ze-2 (10%) azizange zibonise ukuthuthukiswa okuphawulekayo.Ukubuyiselwa kwemilenze, amalunga amadolo, ubuso nentamo kubonakala kakhulu, kuyilapho ukubuyiswa kwamaphethelo kubi.
Nimitha et al.kusetshenziswe ukumiswa kwe-PRP kwamangqamuzana e-epidermal ukuze kulungiswe ukumiswa kanye nokumiswa kwe-phosphate buffer yamangqamuzana e-epidermal ukuze aqhathanise futhi abheke ukubuyiswa kwe-pigment yabo ezigulini ezine-vitiligo ezinzile.Iziguli ezingama-21 ezizinzile ze-vitiligo zafakwa kwatholakala amabala amhlophe angama-42.Isilinganiso sesikhathi esizinzile se-vitiligo sasiyiminyaka engu-4.5.Iningi leziguli libonise ukululama kwe-oval discrete pigment eyindilinga emincane ezinyangeni ezi-1-3 ngemuva kokwelashwa.Phakathi nezinyanga ze-6 zokulandelela, isilinganiso sokutholwa kwe-pigment sasingu-75.6% eqenjini le-PRP kanye ne-65% eqenjini elingelona i-PRP.Umehluko wendawo yokubuyisela umbala phakathi kweqembu le-PRP neqembu elingelona i-PRP lalibaluleke ngokwezibalo.Iqembu le-PRP libonise ukululama okungcono kwe-pigment.Lapho kuhlaziywa izinga lokutholwa kwe-pigment ezigulini ezine-vitiligo ye-segmental, kwakungekho umehluko obalulekile phakathi kweqembu le-PRP neqembu elingelona i-PRP.
Ukusetshenziswa kwe-PRP ku-Chloasma
I-Melasma iwuhlobo lwesifo sobuso esinebala elinemibala, esibonakala kakhulu ebusweni babesifazane abavame ukuchayeka ekukhanyeni kwe-ultraviolet futhi abanombala wesikhumba ojulile.I-pathogenesis yayo ayizange icaciswe ngokugcwele, futhi kunzima ukuyelapha futhi kulula ukuphindeka.Njengamanje, ukwelashwa kwe-chloasma kusebenzisa indlela yokwelapha ehlanganisiwe.Nakuba umjovo ongaphansi kwesikhumba we-PRP unezindlela ezihlukahlukene zokwelashwa kwe-chloasma, ukusebenza kahle kweziguli akwenelisi kakhulu, futhi kulula ukuphindeka emuva ngemva kokuyeka ukwelashwa.Futhi izidakamizwa zomlomo ezifana ne-tranexamic acid ne-glutathione zingase zibangele ukuphazamiseka kwesisu, ukuphazamiseka kokuya esikhathini, ikhanda elibuhlungu, ngisho nokwakheka kwe-deep vein thrombosis.
Ukuhlola ukwelashwa okusha kwe-chloasma kuyisiqondiso esibalulekile ocwaningweni lwe-chloasma.Kubikwa ukuthi i-PRP ingathuthukisa kakhulu izilonda zesikhumba zeziguli ezine-melasma.Cay ı rl ı Et al.ibike ukuthi owesifazane oneminyaka engu-27 ubudala wathola umjovo we-microneedle engaphansi kwe-PRP njalo ezinsukwini eziyi-15.Ekupheleni kokwelashwa kwe-PRP yesithathu, kwaphawulwa ukuthi indawo yokubuyisela i-epidermal pigment yayi> 80%, futhi kwakungekho ukuphindaphinda phakathi nezinyanga ezingu-6.USirithanabadeekul et al.isebenzise i-PRP yokwelapha i-chloasma ukwenza i-RCT eqinile, okwaqinisekisa ngokwengeziwe ukusebenza komjovo we-PRP ongaphakathi kwesikhumba ekwelapheni i-chloasma.
Hofny et al.wasebenzisa indlela ye-immunohistochemical ukuqhuba i-TGF ngokusebenzisa umjovo we-microneedle we-subcutaneous we-PRP ezilonda zesikhumba zeziguli ezine-chloasma nezingxenye ezivamile- β Ukuqhathaniswa kwenkulumo yamaprotheni kubonise ukuthi ngaphambi kokwelashwa kwe-PRP, izilonda zesikhumba zeziguli ezine-chloasma ne-TGF ezungeze izilonda zesikhumba- β Inkulumo yamaprotheni yayiphansi kakhulu kunesikhumba esinempilo (P<0.05).Ngemuva kokwelashwa kwe-PRP, i-TGF yezilonda zesikhumba ezigulini ezine-chloasma- β Inkulumo yamaprotheni yanda kakhulu.Lesi senzakalo sibonisa ukuthi umphumela wokuthuthukisa we-PRP ezigulini ze-chloasma ungafinyelelwa ngokwandisa i-TGF yezilonda zesikhumba- β Inkulumo yamaprotheni ifinyelela umphumela wokwelapha ku-chloasma.
I-Photoelectric Technology Ihlanganiswe ne-Subcutaneous Injection ye-PRP yokwelashwa kwe-Chloasma
Ngokuthuthuka okuqhubekayo kobuchwepheshe be-photoelectric, indima yayo ekwelapheni i-chloasma idonse ukunakwa okwengeziwe kwabacwaningi.Njengamanje, amalaser asetshenziselwa ukwelapha i-chloasma ahlanganisa i-laser eshintshiwe ye-Q, i-lattice laser, ukukhanya okunamandla okushaywa umoya, i-cuprous bromide laser nezinye izindlela zokwelapha.Isimiso siwukuthi ukuqhunyiswa kokukhanya okukhethiwe kwenziwa ngezinhlayiya ze-melanin ngaphakathi noma phakathi kwama-melanocyte ngokukhetha amandla, futhi umsebenzi wama-melanocyte awusebenzi noma uvinjelwe ngamandla aphansi nokuqhuma okuningi kokukhanya, futhi ngesikhathi esifanayo, ukuqhunyiswa okuningi kokukhanya kwezinhlayiya ze-melanin. Kwenziwa, Ingenza izinhlayiya ze-melanin zibe zincane futhi zilungele ukugwinywa futhi zikhishwe ngumzimba.
U-Su Bifeng et al.i-chloasma iphathwe ngomjovo wokukhanya wamanzi we-PRP ohlanganiswe ne-Q switched Nd: YAG 1064nm laser.Phakathi kweziguli ze-100 ezine-chloasma, iziguli ze-15 eqenjini le-PRP + laser zalashwa ngokuyisisekelo, iziguli ze-22 zathuthukiswa kakhulu, iziguli ze-11 zathuthukiswa, kanti isiguli esisodwa sasingasebenzi;Eqenjini le-laser kuphela, amacala ayi-8 eselashwa ngokuyisisekelo, amacala angama-21 asebenza ngokuphawulekayo, amacala ayi-18 athuthukisiwe, futhi amacala ama-3 awazange aphumelele.Umehluko phakathi kwamaqembu amabili wawubalulekile ngokwezibalo (P<0.05).U-Peng Guokai kanye no-Song Jiquan baphinde baqinisekisa ukusebenza kwe-laser eshintshiwe ye-Q ehlanganiswe ne-PRP ekwelapheni i-chloasma yobuso.Imiphumela yabonisa ukuthi i-Q-switched laser ehlanganiswe ne-PRP yayisebenza kahle ekwelapheni i-chloasma ebusweni
Ngokusho kocwaningo lwamanje lwe-PRP kuma-dermatoses ane-pigmented, indlela engenzeka ye-PRP ekwelapheni i-chloasma ukuthi i-PRP ikhulisa i-TGF yezilonda zesikhumba- β Inkulumo yamaprotheni ingathuthukisa iziguli ze-melasma.Ukuthuthukiswa kwe-PRP ezilonda zesikhumba zeziguli ze-vitiligo kungase kuhlobane nama-molecule e-α Adhesion akhiqizwe ama-granules ahlobene nokuthuthukiswa kwemvelo encane yendawo yezilonda ze-vitiligo ngama-cytokines.Ukuqala kwe-vitiligo kuhlobene eduze nokuzivikela okungavamile kwezilonda zesikhumba.Ucwaningo luye lwathola ukuthi ukungahambi kahle kwamasosha omzimba ezigulini ze-vitiligo kuhlobene nokwehluleka kwama-keratinocyte nama-melanocyte ezilonda zesikhumba ukumelana nokulimala kwama-melanocyte okubangelwa izici ezihlukahlukene zokuvuvukala kanye nama-chemokines akhululwa ohlelweni lwe-intracellular oxidative stress.Kodwa-ke, izici ezihlukahlukene zokukhula kwe-platelet ezifihliwe yi-PRP kanye nezinhlobonhlobo zama-cytokines e-anti-inflammatory akhishwe ama-platelet, njenge-soluble tumor necrosis factor receptor I, i-IL-4 ne-IL-10, okungabaphikisi be-interleukin-1 receptor, kungenzeka. ukudlala indima ethile ekulawuleni ibhalansi yamasosha omzimba wendawo yezilonda zesikhumba.
(Okuqukethwe yile ndatshana kuphrintwe kabusha, futhi asinikezeli nganoma yisiphi isiqinisekiso esicacile noma esishiwoyo sokunemba, ukwethembeka noma ukuphelela kokuqukethwe okuqukethwe kulesi sihloko, futhi asinasibopho ngemibono yalesi sihloko, sicela uqonde.)
Isikhathi sokuthumela: Nov-24-2022